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Literature summary extracted from

  • Hidaka, T.; Imai, S.; Hara, O.; Anzai, H.; Murakami, T.; Nagaoka, K.; Seto, H.
    Carboxyphosphoenolpyruvate phosphonomutase, a novel enzyme catalyzing C-P bond formation (1990), J. Bacteriol., 172, 3066-3072.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.8.23 agriculture formation of one of the two C-P bonds in the tripeptide antibiotic bialaphos, a potent herbicide isolated from Streptomyces hygroscopicus SF1293, enzyme is produced in large amounts by high producer strain HP5-29, enzyme may contribute to improvement of bialphos production Streptomyces hygroscopicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.23 mutant NP71 constructed by gene replacement technique Streptomyces hygroscopicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.23 EDTA enzyme is inactive in presence of EDTA, but inhibition is completely relieved by addition of 1 mM MnSO4 or MgSO4 Streptomyces hygroscopicus
5.4.2.9 EDTA similar enzyme Streptomyces hygroscopicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.23 0.25
-
 carboxyphosphoenolpyruvate pH 6.5, 27°C Streptomyces hygroscopicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.23 Mg2+ increases activity, 1-10 mM MgSO4, relative activity 250% Streptomyces hygroscopicus
2.7.8.23 Mn2+ 1 mM MnSO4, relative activity 680%, 10 mM MnSO4, relative activity 600% Streptomyces hygroscopicus
2.7.8.23 additional information NaCl, KCl and NH4(SO4)2 up to 100 mM have no effect Streptomyces hygroscopicus
5.4.2.9 Mg2+ increases activity Streptomyces hygroscopicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.23 32000
-
 2 * 32000, homodimer, SDS-PAGE Streptomyces hygroscopicus
2.7.8.23 56000
-
 gel filtration Streptomyces hygroscopicus
5.4.2.9 32000
-
 2 * 32000, SDS-PAGE, similar enzyme Streptomyces hygroscopicus
5.4.2.9 45000 67000 Streptomyces hygroscopicus SF1293, gel filtration, similar enzyme Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.23 carboxyphosphonoenolpyruvate Streptomyces hygroscopicus biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos (hydroxyphosphinyl)pyruvate + CO2
-
 ?
2.7.8.23 carboxyphosphonoenolpyruvate Streptomyces hygroscopicus SF1293 biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos (hydroxyphosphinyl)pyruvate + CO2
-
 ?
5.4.2.9 phosphoenolpyruvate Streptomyces hygroscopicus enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 3-phosphonopyruvate
-
 ?
5.4.2.9 phosphoenolpyruvate Streptomyces hygroscopicus SF1293 enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 3-phosphonopyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.23 Streptomyces hygroscopicus
-
 HP782, HP5-29 and SF1293 mutant NP71
-
2.7.8.23 Streptomyces hygroscopicus
-
 SF1293
-
2.7.8.23 Streptomyces hygroscopicus SF1293
-
 SF1293
-
5.4.2.9 Streptomyces hygroscopicus
-
 similar enzyme
-
5.4.2.9 Streptomyces hygroscopicus
-
 SF1293
-
5.4.2.9 Streptomyces hygroscopicus
-
 mutant NP71
-
5.4.2.9 Streptomyces hygroscopicus SF1293
-
 SF1293
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.23
-
 Streptomyces hygroscopicus
5.4.2.9 SF1293, mutant NP71 Streptomyces hygroscopicus
5.4.2.9 similar enzyme Streptomyces hygroscopicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.23 mycelium
-
 Streptomyces hygroscopicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.23 additional information
-
 specific activity 217.0 U/mg of protein, one unit is the amount of enzyme required to produce 1micromol of bialaphos Streptomyces hygroscopicus
5.4.2.9 additional information
-
-
 Streptomyces hygroscopicus

Storage Stability

EC Number Storage Stability Organism
2.7.8.23 -20°C, 80% ammonium sulfate suspension, no loss of activity observed for 1 month Streptomyces hygroscopicus
2.7.8.23 4°C, 1 mg/ml in 50 mM MES buffer, pH 6.5, stable for 1 week Streptomyces hygroscopicus
5.4.2.9 -20°C, 80% ammonium sulfate suspension, stable for 1 month, similar enzyme Streptomyces hygroscopicus
5.4.2.9 4°C, 50 mM MES buffer, pH 6.5, 1 mg protein/ml, stable for 1 week, similar enzyme Streptomyces hygroscopicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.23 carboxyphosphoenolpyruvate
-
 Streptomyces hygroscopicus ?
-
 ?
2.7.8.23 carboxyphosphoenolpyruvate
-
 Streptomyces hygroscopicus SF1293 ?
-
 ?
2.7.8.23 carboxyphosphonoenolpyruvate catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate Streptomyces hygroscopicus (hydroxyphosphinyl)pyruvate + CO2
-
 ?
2.7.8.23 carboxyphosphonoenolpyruvate biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos Streptomyces hygroscopicus (hydroxyphosphinyl)pyruvate + CO2
-
 ?
2.7.8.23 carboxyphosphonoenolpyruvate catalyzes the rearrangement of the carboxyphosphonogroup to form the C-P bond of phosphinopyruvate Streptomyces hygroscopicus SF1293 (hydroxyphosphinyl)pyruvate + CO2
-
 ?
2.7.8.23 carboxyphosphonoenolpyruvate biosynthetic pathway that leads to the tripeptide antibiotic bialaphos, formation of one of the two C-P bonds in bialaphos Streptomyces hygroscopicus SF1293 (hydroxyphosphinyl)pyruvate + CO2
-
 ?
5.4.2.9 carboxyphosphoenolpyruvate
-
 Streptomyces hygroscopicus phosphinopyruvate + CO2
-
 ?
5.4.2.9 carboxyphosphoenolpyruvate
-
 Streptomyces hygroscopicus SF1293 phosphinopyruvate + CO2
-
 ?
5.4.2.9 phosphoenolpyruvate enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 Streptomyces hygroscopicus 3-phosphonopyruvate
-
 ?
5.4.2.9 phosphoenolpyruvate enzyme catalyzes the formation of a C-P bond that is involved in the biosynthesis of the antibiotic bialaphos, i.e. SF-1293 Streptomyces hygroscopicus SF1293 3-phosphonopyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.23 dimer 2 * 32000, homodimer, SDS-PAGE Streptomyces hygroscopicus
5.4.2.9 dimer 2 * 32000, SDS-PAGE, similar enzyme Streptomyces hygroscopicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.23 35
-
-
 Streptomyces hygroscopicus
5.4.2.9 35
-
 similar enzyme Streptomyces hygroscopicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.23 6.5
-
-
 Streptomyces hygroscopicus
5.4.2.9 6.5
-
 similar enzyme Streptomyces hygroscopicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.8.23 5.5 8 stable in this range Streptomyces hygroscopicus
5.4.2.9 5.5 8 stable in this range, similar enzyme Streptomyces hygroscopicus